e35 Is pre-methotrexate folate measurement helpful in predicting methotrexate side effects and methotrexate survival?

Methotrexate Side Effects: Review Article

Side effects of MTX high dose (MTX-HD) may be life threatening, however those of various doses of oral MTX are variable because of the interindividual variability of gastrointestinal absorption of this drug. Bone marrow, gastrointestinal mucosa and hair are particularly vulnerable to the effects of MTX, secondary to their high rate of cellular turnover [2] and because MTX concentration is inver...

Rare side effects of subcutaneous methotrexate therapy.

Methotrexate-resistant Leishmania donovani genetically deficient in the folate-methotrexate transporter.

From a mutagenized population of wild type Leishmania donovani promastigotes, a clone was isolated in a single step by virtue of its resistance to 1 mM methotrexate, a potent inhibitor of dihydrofolate reductase. This methotrexate-selected cell line, MTXA5, was cross-resistant to aminopterin but just as sensitive to growth inhibition caused by pyrimethamine, trimethoprim, and cytotoxic purine a...

Methotrexate Pharmacokinetics and Survival in Osteosarcomat

Procedure This study included 44 patients (479 courses) who received a median dose of 5.92 g/m of MTX (interquartile range (IQR) 2.37 g/m) in a 4-hr infusion. The mean area under the concentration–time curve (AUC) estimated by parametric methods (non-parametric expectation maximization, NPEM), and the mean concentration at the end of the infusion were considered to be the exposure parameters. T...

Role of methotrexate polyglutamates in methotrexate- and sequential methotrexate-5-fluorouracil-mediated cell kill.

The synthesis of methotrexate (MTX) polyglutamates has been investigated in a human leukemia cell line, CCRF-CEM. An increase in either the extracellular MTX concentration (0.1-10 microM) or exposure time (4-24 h) allowed a greater accumulation of polyglutamate derivatives. The species which predominated (di-, tri-, or tetraglutamate) was also time and concentration dependent. Derivatives up to...